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KMID : 0861020020170020159
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Korea Journal of Herbology
2002 Volume.17 No. 2 p.159 ~ p.165
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Transformation of Antioxidant Ascorbate Peroxidase Gene
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Abstract
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Objectives : An experiment was carried out to introduce Apx8, ApxSC gene isolated from strawberry to orchardgrass(Dactylis glomerata L.) using Agrobacterium.
Methods : Mature seed-derived calli of orchardgrass were co-cultured with Agrobacterium tumefaciens EHA101 harboring the plasmid pIG-Apx8 for transformation. Calli selected by hygromycin were transferred to N6 medium containing 2 mg/L BAP, 30 g/L sucrose, 0.2 mg/L NAA, 250mg/l cefotaxime and 50mg/l hygromycin and several hygromycin resistant plants were obtained.
Results : Stable incorporation of the introduced Apx8-35 to the genome of the hygromycin resistant plants was confirmed by PCR and Southern blot analysis. Transformation efficiency was variable between cultivates in which it was 13.5% in Potomac and 7.0% in Frontier.
Conclusions : Constitutive expression of the transgene in the transformed orchardgrass tissues was identified by Northern blot analysis but transcript levels were different among individual plants.
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